rabbit anti type 1 collagen col1  (Danaher Inc)

Journal: Bone & Joint Research

Article Title: High-intensity running exercise promotes knee meniscal damage via the PI3K/AKT/mTOR axis

doi: 10.1302/2046-3758.1411.BJR-2024-0535.R1

Figure Lengend Snippet: Excessive mechanical stress promotes meniscus degeneration.a) Immunohistochemical staining revealed the expression of Aggrecan and matrix metalloproteinase 13 (MMP13) in the anterior horn, posterior horn, and articular surface of the meniscus following treadmill intervention in mice. Scale bar: 100 µm. (The same group showed serial sections from the same mouse knee joint tissue for staining analysis.) b) Statistical analysis of the differences in MMP13 and Aggrecan expression between the anterior and posterior horns of the meniscus and the articular cartilage surface groups (n = 5/group). Data were analyzed using one-way analysis of variance (ANOVA) followed by Tukey's post-hoc test. c) Immunofluorescence displaying COL1-positive, Aggrecan-positive, MMP13-positive, and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5)-positive cells as a proportion of total meniscal cells in primary medial meniscal cells (n = 3/group). Data are presented as the mean (SD). Scale bar: 200 µm. d) Quantification of the positive meniscus cells (P-PI3K, P-AKT, and P-S6) as a proportion of total meniscal cells (n = 3/group). Data were analyzed using independent-samples t -test. *p < 0.05, **p < 0.01, ***p < 0.001. ns, non-significant.

Article Snippet: Mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:10,000, ab8245; Abcam, UK), rabbit anti-MMP13 (1:2,000, 18165-1-AP; Proteintech, USA), rabbit anti-A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) (1:1,000, A2836; Abclonal, USA), rabbit anti-collagen type I (COL1) (1:2,000, 14695-1-AP; Proteintech), rabbit anti-Aggrecan (1:1,000, A11691; Abclonal), rabbit anti-phospho-PI3K p85 (1:1,000, YP0224; ImmunoWay, USA), rabbit anti-phospho-AKT (Thr308) (1:500, PC2720; Abmart, China), rabbit anti-phospho-S6 (S240/S244) (1:500, AP0537; Abclonal), rabbit anti-PI3K (1:500, A17433; Abclonal), rabbit anti-AKT (1:1,000, ab192623; Abcam), and rabbit anti-S6 (1:500, A6058; Abclonal) served as the primary antibodies used.

Techniques: Immunohistochemical staining, Staining, Expressing, Immunofluorescence

Journal: World Journal of Stem Cells

Article Title: Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

doi: 10.4252/wjsc.v16.i3.267

Figure Lengend Snippet: The bone density decreased, and the alveolar bone height decreased on the compression side. A: Representative pictures of the rat tooth movement model and 3D model images reconstructed with Mimics software (left) and the statistical diagram of the tooth movement distance (right); B: Representative images of 3D models reconstructed from micro-computed tomography images showing the distance from the alveolar crest to the cemento-enamel junction (left) and the corresponding statistical analysis (right); C: Representative region of interest (ROI) selection diagrams (left) and statistical analysis (right). A certain volume of alveolar bone on the compressed side of the middle 1/2 root of the distal buccal root of the first molar was selected for subsequent analysis. The statistical analysis of the BV/TV, Tb.Th, Tb.N, Tb.Sp and BS/BV values of the ROI region was performed with Siemens software; D: Representative images of hematoxylin and eosin staining; E: Representative images of immunohistochemical staining for type 1 collagen and osteocalcin (left) and quantitative analyses (right); F: Representative images of tartrate-resistant acid phosphatase (TRAP) staining (left) and counting of TRAP-positive multinuclear (> 3 nuclei) cells (right). a P < 0.05 vs control group, b P < 0.01 vs control group. CEJ: Cementum-enamel boundary; HE: Hematoxylin and eosin; TRAP: Tartrate-resistant acid phosphatase; COL1: Type 1 collagen; OCN: Osteocalcin; CT: Computed tomography.

Article Snippet: The primary antibodies used were rabbit anti-type 1 collagen (COL1) and anti-osteocalcin (OCN) (1:500; Abcam, Cambridge, Massachusetts, United States), rabbit anti-ALP (1:500; Invitrogen), and mouse anti-GAPDH (1:1000; ProteinTech, Cook, Illinois, United States).

Techniques: Software, Micro-CT, Selection, Staining, Immunohistochemical staining, Computed Tomography

Journal: World Journal of Stem Cells

Article Title: Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

doi: 10.4252/wjsc.v16.i3.267

Figure Lengend Snippet: Static pressure inhibits the osteogenic differentiation of bone marrow mesenchymal stem cells in vitro . A and B: Representative alkaline phosphatase (ALP) staining images and statistical analysis; C and D: Representative alizarin red staining images and quantitative data from the alirazin red assay; E: mRNA expression of ALP, type 1 collagen (COL1), runt-related transcription factor 2, and osteocalcin (OCN) folded to control group by quantitative reverse transcription-polymerase chain reaction; F: COL1, ALP, and OCN protein expression in bone marrow mesenchymal stem cells analyzed by Western blotting (left), and the band intensities measured with ImageJ software (right). a P < 0.05 vs control group, b P < 0.01 vs control group. ALP: Alkaline phosphatase; RUNX2: Runt-related transcription factor 2; COL1: Type 1 collagen; OCN: Osteocalcin; ARS: Alizarin red staining.

Article Snippet: The primary antibodies used were rabbit anti-type 1 collagen (COL1) and anti-osteocalcin (OCN) (1:500; Abcam, Cambridge, Massachusetts, United States), rabbit anti-ALP (1:500; Invitrogen), and mouse anti-GAPDH (1:1000; ProteinTech, Cook, Illinois, United States).

Techniques: In Vitro, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Software

Journal: World Journal of Stem Cells

Article Title: Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

doi: 10.4252/wjsc.v16.i3.267

Figure Lengend Snippet: Low-intensity pulsed ultrasound promotes the osteogenesis of bone marrow mesenchymal stem cells. A and B: Representative alkaline phosphatase (ALP) staining images and statistical analysis; C and D: Representative alizarin red staining images and quantitative data from the alirazin red assay; E: mRNA expression of ALP, type 1 collagen (COL1), runt-related transcription factor 2, and osteocalcin (OCN) folded to control group by quantitative reverse transcription-polymerase chain reaction; F: COL1, ALP, and OCN protein expression in rat alveolar bone tissues analyzed via Western blotting (left), and the band intensities measured via ImageJ software (right). a P < 0.05 vs control group, b P < 0.01 vs control group. ALP: Alkaline phosphatase; RUNX2: Runt-related transcription factor 2; COL1: Type 1 collagen; OCN: Osteocalcin; LIPUS: Low-intensity pulsed ultrasound; ARS: Alizarin red staining.

Article Snippet: The primary antibodies used were rabbit anti-type 1 collagen (COL1) and anti-osteocalcin (OCN) (1:500; Abcam, Cambridge, Massachusetts, United States), rabbit anti-ALP (1:500; Invitrogen), and mouse anti-GAPDH (1:1000; ProteinTech, Cook, Illinois, United States).

Techniques: Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Software

Journal: World Journal of Stem Cells

Article Title: Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

doi: 10.4252/wjsc.v16.i3.267

Figure Lengend Snippet: Low-intensity pulsed ultrasound promotes bone formation. A: Representative images of hematoxylin and eosin staining; B: Representative images of immunohistochemical staining for type 1 collagen and osteocalcin and tartrate-resistant acid phosphatase staining (TRAP); C-E: Statistical analyses of the immunohistochemical staining and TRAP staining results. a P < 0.05 vs control group, b P < 0.01 vs control group. HE: Hematoxylin and eosin; TRAP: Tartrate-resistant acid phosphatase; COL1: Type 1 collagen; OCN: Osteocalcin; LIPUS: Low-intensity pulsed ultrasound.

Article Snippet: The primary antibodies used were rabbit anti-type 1 collagen (COL1) and anti-osteocalcin (OCN) (1:500; Abcam, Cambridge, Massachusetts, United States), rabbit anti-ALP (1:500; Invitrogen), and mouse anti-GAPDH (1:1000; ProteinTech, Cook, Illinois, United States).

Techniques: Staining, Immunohistochemical staining

Journal: World Journal of Stem Cells

Article Title: Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

doi: 10.4252/wjsc.v16.i3.267

Figure Lengend Snippet: siYAP1 blocks the effect of low-intensity pulsed ultrasound-induced osteogenic differentiation of bone marrow mesenchymal stem cells. A: Representative immunofluorescence images of Yes-associated protein (YAP1) (red) and DAPI (blue) staining (left) and statistical analyses (right); B: Western blotting results for type 1 collagen, YAP1, runt-related transcription factor 2 (RUNX2), and alkaline phosphatase (ALP); C: mRNA expression of ALP, RUNX2, and YAP1 in the force group by quantitative reverse transcription-polymerase chain reaction; D and E: Representative ALP and alizarin red staining staining images and statistical analysis. a P < 0.05 vs control group, b P < 0.01 vs control group. ALP: Alkaline phosphatase; COL1: Type 1 collagen; LIPUS: Low-intensity pulsed ultrasound; RUNX2: Runt-related transcription factor 2; YAP1: Yes-associated protein; ARS: Alizarin red staining.

Article Snippet: The primary antibodies used were rabbit anti-type 1 collagen (COL1) and anti-osteocalcin (OCN) (1:500; Abcam, Cambridge, Massachusetts, United States), rabbit anti-ALP (1:500; Invitrogen), and mouse anti-GAPDH (1:1000; ProteinTech, Cook, Illinois, United States).

Techniques: Immunofluorescence, Staining, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: World Journal of Stem Cells

Article Title: Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

doi: 10.4252/wjsc.v16.i3.267

Figure Lengend Snippet: Putative mechanism of the effect of low-intensity pulsed ultrasound on alveolar bone during orthodontic treatment. Low-intensity pulsed ultrasound has little effect on osteoclast differentiation but may rescue osteogenic gene expression suppressed by static force and promote osteoblastic differentiation by reordering the cytoskeleton, upregulating the expression of F-actin and Lamin A/C, and increasing the nuclear translocation of Yes-associated protein 1, thereby contributing to alveolar bone homeostasis and morphology while accelerating tooth movement during orthodontic treatment. COL1: Type 1 collagen; LIPUS: Low-intensity pulsed ultrasound; RUNX2: Runt-related transcription factor 2; BMSC: Bone marrow mesenchymal stem cell; YAP1: Yes-associated protein.

Article Snippet: The primary antibodies used were rabbit anti-type 1 collagen (COL1) and anti-osteocalcin (OCN) (1:500; Abcam, Cambridge, Massachusetts, United States), rabbit anti-ALP (1:500; Invitrogen), and mouse anti-GAPDH (1:1000; ProteinTech, Cook, Illinois, United States).

Techniques: Expressing, Translocation Assay

Journal: iScience

Article Title: Targeting endometrial inflammation in intrauterine adhesion ameliorates endometrial fibrosis by priming MSCs to secrete C1INH

doi: 10.1016/j.isci.2023.107201

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Col1 , Proteintech , Cat# 14695-1-AP; RRID: AB_2082037.

Techniques: Staining, Recombinant, Cell Counting, Enzyme-linked Immunosorbent Assay, Western Blot, Software

Journal: Stem Cell Research & Therapy

Article Title: C/EBPα regulates the fate of bone marrow mesenchymal stem cells and steroid-induced avascular necrosis of the femoral head by targeting the PPARγ signalling pathway

doi: 10.1186/s13287-022-03027-3

Figure Lengend Snippet: Establishment and evaluation of SANFH rat models. a Scheme of animal treatments. b The coronal (COR), transverse (TRA) and sagittal (SAG) sections of the femoral head were reconstructed by the micro-CT images in the control and model groups. c Quantitative analysis of related parameters of micro-CT. d H&E staining of the femoral head after decalcification, with black arrows indicating trabecular bone, red arrows indicating fat vacuoles, and yellow arrows indicating empty bone lacunae. e, f IHC staining of COL1 in the femoral head and quantitative analysis showed the IOD in the control and model groups. g The expression levels of femoral head-related proteins in the control and model groups were detected by western blotting, and β-actin was used for normalization and quantitative analysis by ImageJ. *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: The remaining femoral head sections were dewaxed, antigen recovered, incubated with the primary antibody (rabbit polyclonal anti-type 1 collagen (COL1) and anti-PPARγ, Proteintech Group, Inc.), and then incubated with the appropriate horseradish peroxide-coupled secondary antibody.

Techniques: Micro-CT, Control, Staining, Immunohistochemistry, Expressing, Western Blot

Journal: Molecular Medicine Reports

Article Title: Interleukin-10 promotes proliferation and migration, and inhibits tendon differentiation via the JAK/Stat3 pathway in tendon-derived stem cells in vitro

doi: 10.3892/mmr.2018.9547

Figure Lengend Snippet: Primers used for reverse transcription-quantitative polymerase chain reaction.

Article Snippet: Cells were then incubated overnight at 4°C with primary rabbit anti-Col1 antibodies (1:100; cat. no. 14695-1-AP; ProteinTech Group, Inc., Chicago, IL, USA) and subsequently incubated with tetramethylrhodamine-tagged goat anti-rabbit IgG secondary antibodies (1:100; cat. no. HA1016; Hangzhou HuaAn Biotechnology Co., Ltd., Hangzhou, China) for 2 h at room temperature.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Interleukin-10 promotes proliferation and migration, and inhibits tendon differentiation via the JAK/Stat3 pathway in tendon-derived stem cells in vitro

doi: 10.3892/mmr.2018.9547

Figure Lengend Snippet: IL-10 inhibits spontaneous tenogenic differentiation. Tendon-derived stem cells were treated with the indicated concentrations of IL-10 for 3 days and subjected to quantitative polymerase chain reaction to detect the gene expression of (A) Scx and (B) Col1. (C) Representative images of the morphological alterations in control and IL-10-treated TDSCs (magnification, ×100). Data are expressed as the mean ± standard error of the mean. *P<0.05, **P<0.01 vs. control cells. IL-10, interleukin-10; Col1, collagen type 1; Scx, scleraxis.

Article Snippet: Cells were then incubated overnight at 4°C with primary rabbit anti-Col1 antibodies (1:100; cat. no. 14695-1-AP; ProteinTech Group, Inc., Chicago, IL, USA) and subsequently incubated with tetramethylrhodamine-tagged goat anti-rabbit IgG secondary antibodies (1:100; cat. no. HA1016; Hangzhou HuaAn Biotechnology Co., Ltd., Hangzhou, China) for 2 h at room temperature.

Techniques: Derivative Assay, Real-time Polymerase Chain Reaction, Gene Expression, Control

Journal: Molecular Medicine Reports

Article Title: Interleukin-10 promotes proliferation and migration, and inhibits tendon differentiation via the JAK/Stat3 pathway in tendon-derived stem cells in vitro

doi: 10.3892/mmr.2018.9547

Figure Lengend Snippet: IL-10 alters the mRNA and protein expression levels tendon-associated molecules in TDSCs. (A) The mRNA expression Tnmd, Col3, Mkx, Egr1, Fmod and Lum was altered by IL-10 treatment. (B) Fluorescent staining of Col1 expression following treatment with IL-10 for 3 days. (C) Rat TDSCs were treated with the indicated concentrations of IL-10 for 3 days and subjected to western blot analysis for Scx, Col1, Tnmd and Col3 protein expression. (D) Densitometric analysis of the western blotting results. Data are expressed as the mean ± standard error of the mean. *P<0.05, **P<0.01 vs. control cells. IL-10, interleukin-10; TDSCs, tendon-derived stem cells; Tnmd, tenomodulin; Col3, collagen type 3; Mkx, mohawk; Egr1, early growth response gene 1; Fmod, fibromodulin; Lum, lumican; Dcn, decorin; Bgn, biglycan.

Article Snippet: Cells were then incubated overnight at 4°C with primary rabbit anti-Col1 antibodies (1:100; cat. no. 14695-1-AP; ProteinTech Group, Inc., Chicago, IL, USA) and subsequently incubated with tetramethylrhodamine-tagged goat anti-rabbit IgG secondary antibodies (1:100; cat. no. HA1016; Hangzhou HuaAn Biotechnology Co., Ltd., Hangzhou, China) for 2 h at room temperature.

Techniques: Expressing, Staining, Western Blot, Control, Derivative Assay

Journal: Molecular Medicine Reports

Article Title: Interleukin-10 promotes proliferation and migration, and inhibits tendon differentiation via the JAK/Stat3 pathway in tendon-derived stem cells in vitro

doi: 10.3892/mmr.2018.9547

Figure Lengend Snippet: IL-10 activates Stat3 but has no effect on Akt expression in TDSCs. (A) Rat TDSCs were treated with the indicated concentrations of IL-10 and were subjected to western blot analysis to detect p-Stat3, Stat3, p-Akt and Akt protein expression. (B) TDSCs were treated with the indicated concentrations of IL-10 with or without the Stat3 inhibitor WP1066 and subjected to western blot analysis for Col1, Tnmd and Col3 protein expression. Data are expressed as the mean ± standard error of the mean. *P<0.05, **P<0.01 vs. 10 ng/ml IL-10. IL-10, interleukin-10; TDSCs, tendon-derived stem cells; p-, phosphorylated; Stat3, Signal transducer and activator of transcription 3; Akt, protein kinase B; Tnmd, tenomodulin; Col3, collagen type 3.

Article Snippet: Cells were then incubated overnight at 4°C with primary rabbit anti-Col1 antibodies (1:100; cat. no. 14695-1-AP; ProteinTech Group, Inc., Chicago, IL, USA) and subsequently incubated with tetramethylrhodamine-tagged goat anti-rabbit IgG secondary antibodies (1:100; cat. no. HA1016; Hangzhou HuaAn Biotechnology Co., Ltd., Hangzhou, China) for 2 h at room temperature.

Techniques: Expressing, Western Blot, Derivative Assay

Journal: PLoS ONE

Article Title: CXCL12/CXCR4 Axis Activation Mediates Prostate Myofibroblast Phenoconversion through Non-Canonical EGFR/MEK/ERK Signaling

doi: 10.1371/journal.pone.0159490

Figure Lengend Snippet: ( A ) N1 fibroblasts were treated with vehicle (DMSO) or EGFR inhibitor (AG1478, 500 nM) prior to CXCL12 treatment. Phosphorylation of EGFR, Akt, Smad and ERK1/2 were assessed via western blot. ( B ) qRT-PCR analysis of myofibroblast marker expression after CXCL12 (100pM) treatment in the presence or absence of AG1478 (500 nM). Expression levels of α-smooth muscle actin (ACTA2) and collagen 1α1 (COL1α1) were analyzed over the course of 24 hours of treatment. Treatment with AG1478 reduced or ablated the CXCL12/CXCR4 -mediated stimulation of both the ACTA2 and COL1α1 genes. ( C ) Western blot analysis of fibroblast markers, α-smooth muscle actin and collagen 1, at 24 and 48 hours after CXCL12 treatment. Secretion and incorporation of collagen 1 was inhibited in the presence of AG1478, α-smooth muscle actin production was inhibited as well. ( D ) Signal intensity quantification for 7 and collagen 1 western blots. * = p-value < 0.05. Error bars, SE.

Article Snippet: Primary antibodies were diluted in blocking solution and included 1:200 dilution FITC-conjugated mouse monoclonal anti-α-smooth muscle actin (α SMA), and 1:100 dilution biotin-conjugated rabbit polyclonal anti-collagen type 1 (COL1) (Rockland Immunochemicals, Gilbertsville, PA).

Techniques: Phospho-proteomics, Western Blot, Quantitative RT-PCR, Marker, Expressing

Journal: PLoS ONE

Article Title: CXCL12/CXCR4 Axis Activation Mediates Prostate Myofibroblast Phenoconversion through Non-Canonical EGFR/MEK/ERK Signaling

doi: 10.1371/journal.pone.0159490

Figure Lengend Snippet: ( A ) Immunofluorescence analysis of N1 fibroblasts left untreated (-CXCL12) or treated with 100pM CXCL12 (CXCL12) for 48 hours in the absence or presence of CXCR4 (250 μM AMD3100), EGFR (250 uM AG1478), ALK-5 (TGFβRII) (20μM A-83-01) small molecular inhibitors or an antibody against TGFβRII (200 ng/ml TGFβ MAb). Figure depicts photomicrographs images of cells stained for α-smooth muscle actin (green) or collagen 1 (red) proteins or DAPI (blue nuclear stain); orange color indicates α-smooth muscle actin and collagen 1 colocalization in the merged image. ( B ) Western blot analysis of fibroblast markers, α-smooth muscle actin and collagen 1 at 24, 48 and 72 hours after CXCL12 (100pM) of scramble, anti-CXCR4, and anti-TGFβRI siRNAs transfected fibroblasts. CXCL12-driven expression of myofibroblasts markers does not require the presence or activation of TGFβRI. ( C ) Quantification of western blot images for myofibroblast markers.

Article Snippet: Primary antibodies were diluted in blocking solution and included 1:200 dilution FITC-conjugated mouse monoclonal anti-α-smooth muscle actin (α SMA), and 1:100 dilution biotin-conjugated rabbit polyclonal anti-collagen type 1 (COL1) (Rockland Immunochemicals, Gilbertsville, PA).

Techniques: Immunofluorescence, Staining, Western Blot, Transfection, Expressing, Activation Assay